Manipulation of Human Mesenchymal Stem Cells by Multifunctional Graphene-PEDOT Microelectrode Arrays

In this study, we report the development of all-solution-processed multifunctional organic bioelectronics, comprising reduced graphene oxide (rGO) and dexamethasone 21-phosphate disodium salt (DEX) drug loaded poly(3,4-ethylenedioxythiophene) (PEDOT) microelectrode arrays on indium tin oxide glass, that can be used to manipulate human mesenchymal stem cell (hMSC) spreading morphology on rGO micropattern arrays and trigger the drug releasing from the PEDOT micropatterns. In our devices, the rGO material were used as the adhesive coating to attract the adhesion of hMSC cells whereas PLL-g-PEG coated PEDOT electrodes served as the anti-adhesive coating where no hMSC cells can attach. In addition, PEDOT microelectrodes also work as drug releasing components where spatial-temporal control of DEX releasing from PEDOT matrix can be achieved for long-term cell culture for inducing the osteogenetic differentiation of hMSCs via cyclic potential stimulation (CPS).